The circular RNA ZNF292 alleviates OGD-induced injury in H9c2 cells via targeting BNIP3.

ABSTRACT: The research aims to explore the roles and regulatory mechanisms of the circular RNA (circRNA) ZNF292 (circZNF292) in OGD-induced damage in H9c2 cells. The H9c2 cells were treated by OGD and/or transfected with circZNF292, si-circZNF292, pc-Bcl-2/adenovirus E1B-19 kDa-interacting protein 3 (BNIP3) or corresponding controls. Cell viability was detected with the CCK-8. The protein expression levels of the Bax, caspase-3, Beclin-1, p62, LC3, BNIP3, Wnt3a, β-catenin and mammalian target of rapamycin (mTOR) were individually determined via western blot. qRT-PCR was used to examine the circZNF292 expression level. The apoptotic rate was determined by the Annexin V-FITC/PI with flow cytometer. The production of the circZNF292 was promoted by OGD. Abundant circZNF292 released OGD-induced damage by

SUBMITTER: Ren Q 

PROVIDER: S-EPMC6927697 | biostudies-literature | 2019 Dec

REPOSITORIES: biostudies-literature