Development of a high yielding expression platform for the introduction of non-natural amino acids in protein sequences.

ABSTRACT: The ability to genetically encode non-natural amino acids (nnAAs) into proteins offers an expanded tool set for protein engineering. nnAAs containing unique functional moieties have enabled the study of post-translational modifications, protein interactions, and protein folding. In addition, nnAAs have been developed that enable a variety of biorthogonal conjugation chemistries that allow precise and efficient protein conjugations. These are being studied to create the next generation of antibody-drug conjugates with improved efficacy, potency, and stability for the treatment of cancer. However, the efficiency of nnAA incorporation, and the productive yields of cell-based expression systems, have limited the utility and widespread use of this technology. We developed a process to isolate s

SUBMITTER: Roy G 

PROVIDER: S-EPMC6927762 | biostudies-literature | 2020 Jan-Dec

REPOSITORIES: biostudies-literature