Dataset Information

High-Throughput Automated Olfactory Phenotyping of Group-Housed Mice.

ABSTRACT: Behavioral phenotyping of mice is often compromised by manual interventions of the experimenter and limited throughput. Here, we describe a fully automated behavior setup that allows for quantitative analysis of mouse olfaction with minimized experimenter involvement. Mice are group-housed and tagged with unique RFID chips. They can freely initiate trials and are automatically trained on a go/no-go task, learning to distinguish a rewarded from an unrewarded odor. Further, odor discrimination tasks and detailed training aspects can be set for each animal individually for automated execution without direct experimenter intervention. The procedure described here, from initial RFID implantation to discrimination of complex odor mixtures at high accuracy, can be completed within <2 months with cohorts of up to 25 male mice. Apart from the presentation of monomolecular odors, the setup can generate arbitrary mixtures and dilutions from any set of odors to create complex stimuli, enabling demanding behavioral analyses at high-throughput.

SUBMITTER: Reinert JK

PROVIDER: S-EPMC6927946 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature

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Publications

High-Throughput Automated Olfactory Phenotyping of Group-Housed Mice.

Reinert Janine K JK Schaefer Andreas T AT Kuner Thomas T

Frontiers in behavioral neuroscience 20191217

Behavioral phenotyping of mice is often compromised by manual interventions of the experimenter and limited throughput. Here, we describe a fully automated behavior setup that allows for quantitative analysis of mouse olfaction with minimized experimenter involvement. Mice are group-housed and tagged with unique RFID chips. They can freely initiate trials and are automatically trained on a go/no-go task, learning to distinguish a rewarded from an unrewarded odor. Further, odor discrimination tas ...[more]

PMID: 31920577

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Project description:BackgroundRestricting transpiration under high vapor pressure deficit (VPD) is a promising water-saving trait for drought adaptation. However, it is often measured under controlled conditions and at very low throughput, unsuitable for breeding. A few high-throughput phenotyping (HTP) studies exist, and have considered only maximum transpiration rate in analyzing genotypic differences in this trait. Further, no study has precisely identified the VPD breakpoints where genotypes restrict transpiration under natural conditions. Therefore, outdoors HTP data (15 min frequency) of a chickpea population were used to automate the generation of smooth transpiration profiles, extract informative features of the transpiration response to VPD for optimal genotypic discretization, identify VPD breakpoints, and compare genotypes.ResultsFifteen biologically relevant features were extracted from the transpiration rate profiles derived from load cells data. Genotypes were clustered (C1, C2, C3) and 6 most important features (with heritability > 0.5) were selected using unsupervised Random Forest. All the wild relatives were found in C1, while C2 and C3 mostly comprised high TE and low TE lines, respectively. Assessment of the distinct p-value groups within each selected feature revealed highest genotypic variation for the feature representing transpiration response to high VPD condition. Sensitivity analysis on a multi-output neural network model (with R of 0.931, 0.944, 0.953 for C1, C2, C3, respectively) found C1 with the highest water saving ability, that restricted transpiration at relatively low VPD levels, 56% (i.e. 3.52 kPa) or 62% (i.e. 3.90 kPa), depending whether the influence of other environmental variables was minimum or maximum. Also, VPD appeared to have the most striking influence on the transpiration response independently of other environment variable, whereas light, temperature, and relative humidity alone had little/no effect.ConclusionThrough this study, we present a novel approach to identifying genotypes with drought-tolerance potential, which overcomes the challenges in HTP of the water-saving trait. The six selected features served as proxy phenotypes for reliable genotypic discretization. The wild chickpeas were found to limit water-loss faster than the water-profligate cultivated ones. Such an analytic approach can be directly used for prescriptive breeding applications, applied to other traits, and help expedite maximized information extraction from HTP data.

Dataset Information

High-Throughput Automated Olfactory Phenotyping of Group-Housed Mice.

Publications

High-Throughput Automated Olfactory Phenotyping of Group-Housed Mice.

Similar Datasets

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