Cell shape determines gene expression: cardiomyocyte morphotypic transcriptomes.

ABSTRACT: Cardiomyocytes undergo considerable changes in cell shape. These can be due to hemodynamic constraints, including changes in preload and afterload conditions, or to mutations in genes important for cardiac function. These changes instigate significant changes in cellular architecture and lead to the addition of sarcomeres, at the same time or at a later stage. However, it is currently unknown whether changes in cell shape on their own affect gene expression and the aim of this study was to fill that gap in our knowledge. We developed a single-cell morphotyping strategy, followed by single-cell RNA sequencing, to determine the effects of altered cell shape in gene expression. This enabled us to profile the transcriptomes of individual cardiomyocytes of defined geometrical morphotypes and ch

SUBMITTER: Haftbaradaran Esfahani P 

PROVIDER: S-EPMC6928094 | biostudies-literature | 2019 Dec

REPOSITORIES: biostudies-literature