Competition between Granule Bound Starch Synthase and Starch Branching Enzyme in Starch Biosynthesis.

ABSTRACT:

Background

Starch branching enzymes (SBE) and granule-bound starch synthase (GBSS) are two important enzymes for starch biosynthesis. SBE mainly contributes to the formation of side branches, and GBSS mainly contributes for the synthesis of amylose molecules. However, there are still gaps in the understanding of possible interactions between SBE and GBSS.

Results

Nineteen natural rice varieties with amylose contents up to 28% were used. The molecular structure, in the form of the chain-length distribution (CLDs, the distribution of the number of monomer units in each branch) was measured after enzymatic debranching, using fluorophore-assisted carbohydrate electrophoresis for amylopectin and size- exclusion chromatography for amylose. The resulting distributions were fitted t

SUBMITTER: Han H 

PROVIDER: S-EPMC6928174 | biostudies-literature | 2019 Dec

REPOSITORIES: biostudies-literature