Molecular Detection and Differentiation of Theileria lestoquardi, T. ovis and T. annulata in Blood of Goats and Ticks in Kermanshah Province, Iran.

ABSTRACT:

Background

This study was carried out to identify Theileria spp. infections in goats and ticksin Kermanshah Province, western Iran from May-Sep 2015.

Methods

For differentiation of different Theileria spp. both blood and tick samples were examined by nested PCR-RFLP.

Results

Light microscopy of blood smears revealed Theileria spp. infection in 22 (5.5%), while 68 (17%) of blood samples were positive using nested PCR. Out of 68 positive samples, 85.3% (58/68) and 11.7% (8/68) were respectively positive for Theileria ovis and T. lestoquardi. Mixed infection was detected in 3% (2/68) cases. Overall, 420 ixodid ticks belong to seven different hard ticks species were collected from goats. Rhipicephalus turanicus 112 (26.7%), R.

SUBMITTER: Rahmani-Varmale M 

PROVIDER: S-EPMC6928382 | biostudies-literature | 2019 Sep

REPOSITORIES: biostudies-literature