A comparison framework and guideline of clustering methods for mass cytometry data.

ABSTRACT:

Background

With the expanding applications of mass cytometry in medical research, a wide variety of clustering methods, both semi-supervised and unsupervised, have been developed for data analysis. Selecting the optimal clustering method can accelerate the identification of meaningful cell populations.

Result

To address this issue, we compared three classes of performance measures, "precision" as external evaluation, "coherence" as internal evaluation, and stability, of nine methods based on six independent benchmark datasets. Seven unsupervised methods (Accense, Xshift, PhenoGraph, FlowSOM, flowMeans, DEPECHE, and kmeans) and two semi-supervised methods (Automated Cell-type Discovery and Classification and linear discriminant analysis (LDA)) are tested on six mass cytometry

SUBMITTER: Liu X 

PROVIDER: S-EPMC6929440 | biostudies-literature | 2019 Dec

REPOSITORIES: biostudies-literature