Soluble versions of outer membrane cytochromes function as exporters for heterologously produced cargo proteins.

ABSTRACT: This study reveals that it is possible to secrete truncated versions of outer membrane cytochromes into the culture supernatant and that these proteins can provide a basis for the export of heterologously produced proteins. Different soluble and truncated versions of the outer membrane cytochrome MtrF were analyzed for their suitability to be secreted. A protein version with a very short truncation of the N-terminus to remove the recognition sequence for the addition of a lipid anchor is secreted efficiently to the culture supernatant, and moreover this protein could be further truncated by a deletion of 160 amino acid and still is detectable in the supernatant. By coupling a cellulase to this soluble outer membrane cytochrome, the export efficiency was measured by means of relative cellul

SUBMITTER: Dietrich HM 

PROVIDER: S-EPMC6929479 | biostudies-literature | 2019 Dec

REPOSITORIES: biostudies-literature