Human primary middle ear epithelial cell culture: A novel in vitro model to study otitis media.

ABSTRACT:

Objectives

Otitis media (OM) is a ubiquitous pediatric disease leading to a significant health care burden. There is no medication beneficial to resolving COM fluid, highlighting the need for research in the field. Crucially, current human middle ear epithelial cell models are transformed cells not recapitulating physiological functions. Herein, we describe a new method to proliferate and differentiate pediatric primary middle ear epithelial cells (pMEEC) from patients as a physiological model for the study of OM.

Methods

We adapted a cell reprogramming protocol using irradiated fibroblast feeder medium in addition to Rho kinase inhibitor to proliferate pMEEC collected during cochlear implant surgery. Cells were plated on transwell membranes, proliferated with conditionally

SUBMITTER: Chen Y 

PROVIDER: S-EPMC6929573 | biostudies-literature | 2019 Dec

REPOSITORIES: biostudies-literature