Fusobacterium nucleatum Promotes Metastasis in Colorectal Cancer by Activating Autophagy Signaling via the Upregulation of CARD3 Expression.

ABSTRACT: Aims: We aimed to measure the abundance of Fusobacterium nucleatum (F. nucleatum) in colorectal cancer (CRC) tissues from patients and to uncover the function of this bacterium in colorectal tumor metastasis. Methods: We collected metastatic and non-metastatic CRC tissues to analyze F. nucleatum abundance. Cells were incubated with F. nucleatum or chloroquine (CQ) or were transfected with CARD3-targeting siRNA; the expression of mRNAs and proteins was then measured. CRC cells stably transfected with shRNA-luc were mixed with F. nucleatum and intravenously injected into BALB/cJ mice. APC^Min/+, CARD3^-/-and CARD3^wt C57BL mice were given F. nucleatum; some mice were given azoxymethane (AOM) and dextran sod

SUBMITTER: Chen Y 

PROVIDER: S-EPMC6929621 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature