Simultaneous detection of genotype and phenotype enables rapid and accurate antibiotic susceptibility determination.

ABSTRACT: Multidrug resistant organisms are a serious threat to human health^1,2. Fast, accurate antibiotic susceptibility testing (AST) is a critical need in addressing escalating antibiotic resistance, since delays in identifying multidrug resistant organisms increase mortality^3,4 and use of broad-spectrum antibiotics, further selecting for resistant organisms. Yet current growth-based AST assays, such as broth microdilution⁵, require several days before informing key clinical decisions. Rapid AST would transform the care of patients with infection while ensuring that our antibiotic arsenal is deployed as efficiently as possible. Growth-based assays are fundamentally constrained in speed by doubling time of the pathogen, and genotypic assays are limited by the ever-

SUBMITTER: Bhattacharyya RP 

PROVIDER: S-EPMC6930013 | biostudies-literature | 2019 Dec

REPOSITORIES: biostudies-literature