Restricted myeloperoxidase epitopes drive the adaptive immune response in MPO-ANCA vasculitis.

ABSTRACT:

Background

Treatment of autoimmune diseases has relied on broad immunosuppression. Knowledge of specific interactions between human leukocyte antigen (HLA), the autoantigen, and effector immune cells, provides the foundation for antigen-specific therapies. These studies investigated the role of HLA, specific myeloperoxidase (MPO) epitopes, CD4⁺ T cells, and ANCA specificity in shaping the immune response in patients with anti-neutrophil cytoplasmic autoantibody (ANCA) vasculitis.

Methods

HLA sequence-based typing identified enriched alleles in our patient population (HLA-DPB1*04:01 and HLA-DRB4*01:01), while in silico and in vitro binding studies confirmed binding between HLA and specific MPO epitopes. Class II tetramers with MPO peptides were utilized to detect a

SUBMITTER: Free ME 

PROVIDER: S-EPMC6930338 | biostudies-literature | 2020 Jan

REPOSITORIES: biostudies-literature