LncRNA FOXD1-AS1 acts as a potential oncogenic biomarker in glioma.

ABSTRACT:

Aims

Altered activities of long noncoding RNAs (lncRNAs) have been associated with cancer development, and lncRNA FOXD1-AS1 (FOXD1-AS1) is the antisense transcript of the gene encoding for FOXD1, known for its role as an oncogene in several tumor types including glioma. However, the role of FOXD1-AS1 in the differentiation and progression of glioma is not well known.

Methods

Expression profile chip and qPCR were used to screen and identify FOXD1-AS1. Glioma cells were transfected with siRNA or eukaryotic expression vector to observe FOXD1-AS1 function in vitro and in vivo. Dual luciferase reporter gene analysis, Western blot, and ChIRP-MS were used to detect microRNAs and protein that combine with FOXD1-AS1.

Results

FOXD1-AS1 was upregulated and directly correlated wi

SUBMITTER: Gao YF 

PROVIDER: S-EPMC6930828 | biostudies-literature | 2020 Jan

REPOSITORIES: biostudies-literature