Characterization of inflammation and insulin resistance in high-fat diet-induced male C57BL/6J mouse model of obesity.

ABSTRACT:

Background

Animal models of diet-induced obesity (DIO) are commonly used in medical research for mimicking human diseases. There is no universal animal model, and careful evaluation of variety of factors needs to be considered when designing new experiments. Here, we investigated the effect of 9 weeks high-fat diet (HFD) intervention, providing 60% energy from fat, on parameters of inflammation and insulin resistance in male C57BL/6J mice.

Methods

Six weeks old mice were initiated on regular diet (RD) or HFD providing 60 kcal energy from fat for 9 weeks. Fasting blood glucose levels were measured by glucometer, and fasting plasma levels of insulin and proinflammatory cytokines by Luminex assay. Insulin sensitivity was evaluated by using QUICKI and HOMA2 indexes.

Results</

SUBMITTER: Avtanski D 

PROVIDER: S-EPMC6930989 | biostudies-literature | 2019 Dec

REPOSITORIES: biostudies-literature