Local synthesis of hepcidin in the anterior segment of the eye: A novel observation with physiological and pathological implications.

ABSTRACT:

Purpose

The avascular cornea, trabecular meshwork (TM), and lens obtain iron, an essential biometal, from the aqueous humor (AH). The mechanism by which this exchange is regulated, however, is unclear. Recently we reported that non-pigmented ciliary epithelial cells express ferroportin (Fpn) (Ashok, 2018b), an iron export protein modulated by hepcidin, the master regulator of iron homeostasis secreted mainly by the liver. Here, we explored whether ciliary epithelial and other cells in the anterior segment synthesize hepcidin, suggesting local regulation of iron exchange at this site.

Methods

Human and bovine eyes were dissected to isolate the ciliary body (CB), corneal endothelial (CE), TM, lens epithelial (LE), and outer epithelial cell layer of the iris. Total mRNA and pro

SUBMITTER: Ashok A 

PROVIDER: S-EPMC6931014 | biostudies-literature | 2020 Jan

REPOSITORIES: biostudies-literature