Virus-induced gene silencing (VIGS) in Cannabis sativa L.

ABSTRACT:

Background

The raised demand of cannabis as a medicinal plant in recent years led to an increased interest in understanding the biosynthetic routes of cannabis metabolites. Since there is no established protocol to generate stable gene knockouts in cannabis, the use of a virus-induced gene silencing (VIGS) method, resulting in a gene knockdown, to study gene functions is desirable.

Results

For this, a computational approach was employed to analyze the Cannabis sativa L. transcriptomic and genomic resources. Reporter genes expected to give rise to easily scorable phenotypes upon silencing, i.e. the phytoene desaturase (PDS) and magnesium chelatase subunit I (ChlI), were identified in C. sativa. Subsequently, the targets of specific sm

SUBMITTER: Schachtsiek J 

PROVIDER: S-EPMC6931244 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature