Novel pharmacological actions of trequinsin hydrochloride improve human sperm cell motility and function.

ABSTRACT:

Background and purpose

Asthenozoospermia is a leading cause of male infertility, but development of pharmacological agents to improve sperm motility is hindered by the lack of effective screening platforms and knowledge of suitable molecular targets. We have demonstrated that a high-throughput screening (HTS) strategy and established in vitro tests can identify and characterise compounds that improve sperm motility. Here, we applied HTS to identify new compounds from a novel small molecule library that increase intracellular calcium ([Ca²⁺ ]_i ), promote human sperm cell motility, and systematically determine the mechanism of action.

Experimental approach

A validated HTS fluorometric [Ca²⁺ ]_i assay was used to screen an in-house lib

SUBMITTER: McBrinn RC 

PROVIDER: S-EPMC6932944 | biostudies-literature | 2019 Dec

REPOSITORIES: biostudies-literature