Molecular profiling of the basement membrane of pluripotent epiblast cells in post-implantation stage mouse embryos.

ABSTRACT:

Introduction

The basement membrane (BM) is a sheet-like extracellular matrix (ECM) lining the basal side of epithelial and endothelial cells. The molecular composition of the BM diversifies as embryonic development proceeds, providing optimized microenvironments for individual cell types. In post-implantation stage embryos, the embryonic BMs are essential for differentiation of the epiblast, a layer of multipotent embryonic stem cells, and subsequent embryogenesis. To better understand the role of BMs and cell-BM interactions in early embryogenesis, it is imperative to accumulate information on the molecular entities of the embryonic BMs.

Methods

We analyzed the expressions and localizations of 20 major BM proteins (11 laminin subunits, 6 type IV collagen subunits, nidogen-1

SUBMITTER: Futaki S 

PROVIDER: S-EPMC6933449 | biostudies-literature | 2019 Dec

REPOSITORIES: biostudies-literature