Exploring the changing landscape of cell-to-cell variation after CTCF knockdown via single cell RNA-seq.

ABSTRACT: BACKGROUND:CCCTC-Binding Factor (CTCF), also known as 11-zinc finger protein, participates in many cellular processes, including insulator activity, transcriptional regulation and organization of chromatin architecture. Based on single cell flow cytometry and single cell RNA-FISH analyses, our previous study showed that deletion of CTCF binding site led to a significantly increase of cellular variation of its target gene. However, the effect of CTCF on genome-wide landscape of cell-to-cell variation remains unclear. RESULTS:We knocked down CTCF in EL4 cells using shRNA, and conducted single cell RNA-seq on both wild type (WT) cells and CTCF-Knockdown (CTCF-KD) cells using Fluidigm C1 system. Principal component analysis of single cell RNA-seq data showed that WT and CTCF-KD cells concentra

SUBMITTER: Wang W 

PROVIDER: S-EPMC6933653 | biostudies-literature | 2019 Dec

REPOSITORIES: biostudies-literature