Screening for functional IRESes using α-complementation system of β-galactosidase in Pichia pastoris.

ABSTRACT:

Background

Pichia pastoris is becoming a promising chassis cell for metabolic engineering and synthetic biology after its whole genome and transcriptome sequenced. However, the current systems for multigene co-expression in P. pastoris are not efficient. The internal ribosome entry site (IRES) has an ability to recruit the ribosome to initiate protein synthesis by cap-independent translation manner. This study seeks to screen IRES sequences that are functional in P. pastoris, which will allow P. pastoris to express multiple proteins in a single mRNA and increase its efficacy as a platform for metabolic engineering and synthetic biology.

Results

In order to efficiently screen the IRES sequences, we first set out to create a screening system using <

SUBMITTER: Huang Y 

PROVIDER: S-EPMC6933714 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature