NAGbinder: An approach for identifying N-acetylglucosamine interacting residues of a protein from its primary sequence.

ABSTRACT: N-acetylglucosamine (NAG) belongs to the eight essential saccharides that are required to maintain the optimal health and precise functioning of systems ranging from bacteria to human. In the present study, we have developed a method, NAGbinder, which predicts the NAG-interacting residues in a protein from its primary sequence information. We extracted 231 NAG-interacting nonredundant protein chains from Protein Data Bank, where no two sequences share more than 40% sequence identity. All prediction models were trained, validated, and evaluated on these 231 protein chains. At first, prediction models were developed on balanced data consisting of 1,335 NAG-interacting and noninteracting residues, using various window size. The model developed by implementing Random Forest using binary profil

SUBMITTER: Patiyal S 

PROVIDER: S-EPMC6933864 | biostudies-literature | 2020 Jan

REPOSITORIES: biostudies-literature