Cholesterol restricts lymphotoxin β receptor-triggered NF-κB signaling.

ABSTRACT:

Background

Lymphotoxin β receptor (LTβR) plays important roles in the development of the immune system and immune response. At the cellular level, ligand-bound LTβR activates the pro-inflammatory NF-κB pathway but the detailed mechanisms regulating its signaling remain unknown. Understanding them is of high importance since LTβR and its ligands are promising therapeutic targets. Here, we studied the consequences of perturbed cellular cholesterol content on LTβR-induced NF-κB signaling.

Methods

To modulate cholesterol availability and/or level in lung carcinoma A549 and H2228, and endothelial HUVEC cells different treatment regimens with filipin, methyl-β-cyclodextrin and simvastatin were applied. LTβR localization was studied by confocal microscopy. The activity of LTβR-indu

SUBMITTER: Banach-Orlowska M 

PROVIDER: S-EPMC6933913 | biostudies-literature | 2019 Dec

REPOSITORIES: biostudies-literature