Fast and accurate quantification of insertion-site specific transgene levels from raw seed samples using solid-state nanopore technology.

ABSTRACT: Many modern crop varieties contain patented biotechnology traits, and an increasing number of these crops have multiple (stacked) traits. Fast and accurate determination of transgene levels is advantageous for a variety of use cases across the food, feed and fuel value chain. With the growing number of new transgenic crops, any technology used to quantify them should have robust assays that are simple to design and optimize, thereby facilitating the addition of new traits to an assay. Here we describe a PCR-based method that is simple to design, starts from whole seeds, and can be run to end-point in less than 5 minutes. Subsequent relative quantification (trait vs. non-trait) using capillary electrophoresis performed in 5% increments across the 0-100% range showed a mean absolute error of

SUBMITTER: Pearson MD 

PROVIDER: S-EPMC6934305 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature