Multi-strain Tn-Seq reveals common daptomycin resistance determinants in Staphylococcus aureus.

ABSTRACT: Antibiotic-resistant Staphylococcus aureus remains a leading cause of antibiotic resistance-associated mortality in the United States. Given the reality of multi-drug resistant infections, it is imperative that we establish and maintain a pipeline of new compounds to replace or supplement our current antibiotics. A first step towards this goal is to prioritize targets by identifying the genes most consistently required for survival across the S. aureus phylogeny. Here we report the first direct comparison of multiple strains of S. aureus via transposon sequencing. We show that mutant fitness varies by strain in key pathways, underscoring the importance of using more than one strain to differentiate between core and strain-dependent essential genes. We treated the libraries with daptomycin

SUBMITTER: Coe KA 

PROVIDER: S-EPMC6934316 | biostudies-literature | 2019 Nov

REPOSITORIES: biostudies-literature