Proton-transfer pathways in the mitochondrial S. cerevisiae cytochrome c oxidase.

ABSTRACT: In cytochrome c oxidase (CytcO) reduction of O₂ to water is linked to uptake of eight protons from the negative side of the membrane: four are substrate protons used to form water and four are pumped across the membrane. In bacterial oxidases, the substrate protons are taken up through the K and the D proton pathways, while the pumped protons are transferred through the D pathway. On the basis of studies with CytcO isolated from bovine heart mitochondria, it was suggested that in mitochondrial CytcOs the pumped protons are transferred though a third proton pathway, the H pathway, rather than through the D pathway. Here, we studied these reactions in S. cerevisiae CytcO, which serves as a model of the mammalian counterpart. We analyzed the effect of mutations in the D (Asn99Asp a

SUBMITTER: Bjorck ML 

PROVIDER: S-EPMC6934443 | biostudies-literature | 2019 Dec

REPOSITORIES: biostudies-literature