Reversibility of membrane permeabilization upon pulsed electric field treatment in Lactobacillus plantarum WCFS1.

ABSTRACT: Pulsed electric field (PEF) treatment, or electroporation, can be used to load molecules into cells. The permeabilizing effect of the PEF treatment on the cellular membrane can be either reversible or irreversible depending on the severity of the PEF treatment conditions. The influence of PEF on the reversibility of membrane permeabilization in Lactobacillus plantarum WCFS1 by two different fluorescent staining methods was investigated in this study. Whereas staining with propidium iodide (PI) before and after PEF treatment indicated small reversible permeabilized fractions of maximum 14%, the use of a double staining method with PI and SYTOX Green suggested larger reversible permeabilized fractions up to 40% of the population. This difference shows that the choice for a fluorescent staini

SUBMITTER: Vaessen EMJ 

PROVIDER: S-EPMC6934533 | biostudies-literature | 2019 Dec

REPOSITORIES: biostudies-literature