Rheumatoid arthritis patients display B-cell dysregulation already in the naive repertoire consistent with defects in B-cell tolerance.

ABSTRACT: B cells are postulated to be central in seropositive rheumatoid arthritis (RA). Here, we use exploratory mass cytometry (n = 23) and next-generation sequencing (n = 19) to study B-cell repertoire shifts in RA patients. Expression of several B-cell markers were significantly different in ACPA⁺ RA compared to healthy controls, including an increase in HLA-DR across subsets, CD22 in clusters of IgM⁺ B cells and CD11c in IgA⁺ memory. Moreover, both IgA⁺ and IgG⁺ double negative (IgD^- CD27^-) CD11c⁺ B cells were increased in ACPA⁺ RA, and there was a trend for elevation in a CXCR5/CCR6^high transitional B-cell cluster. In the RA BCR repertoire, there were significant differences in subcla

SUBMITTER: Wang Y 

PROVIDER: S-EPMC6934703 | biostudies-literature | 2019 Dec

REPOSITORIES: biostudies-literature