Deep sequencing and automated histochemistry of human tissue slice cultures improve their usability as preclinical model for cancer research.

ABSTRACT: Cancer research requires models closely resembling the tumor in the patient. Human tissue cultures can overcome interspecies limitations of animal models or the loss of tissue architecture in in vitro models. However, analysis of tissue slices is often limited to histology. Here, we demonstrate that slices are also suitable for whole transcriptome sequencing and present a method for automated histochemistry of whole slices. Tumor and peritumoral tissue from a patient with glioblastoma was processed to slice cultures, which were treated with standard therapy including temozolomide and X-irradiation. Then, RNA sequencing and automated histochemistry were performed. RNA sequencing was successfully accomplished with a sequencing depth of 243 to 368 x 10⁶ reads per sample. Comparing

SUBMITTER: Haehnel S 

PROVIDER: S-EPMC6934722 | biostudies-literature | 2019 Dec

REPOSITORIES: biostudies-literature