Development of hypoallergenic variants of the major horse allergen Equ c 1 for immunotherapy by rational structure based engineering.

ABSTRACT: The use of recombinant allergens is a promising approach in allergen-specific immunotherapy (AIT). Considerable limitation, however, has been the ability of recombinant allergens to activate effector cells leading to allergic reactions. Recombinant hypoallergens with preserved protein folding and capacity to induce protective IgG antibodies binding effectively to the native allergen upon sensitization would be beneficial for safer AIT. In this study, hypoallergen variants of the major horse allergen Equ c 1 were designed by introducing one point mutation on the putative IgE epitope region and two mutations on the monomer-monomer interface of Equ c 1 dimer. The recombinant Equ c 1 wild type and the variants were produced and purified to homogeneity, characterized by size-exclusion ultra-hig

SUBMITTER: Haka J 

PROVIDER: S-EPMC6934807 | biostudies-literature | 2019 Dec

REPOSITORIES: biostudies-literature