A ligation-based single-stranded library preparation method to analyze cell-free DNA and synthetic oligos.

ABSTRACT:

Background

Cell-free DNA (cfDNA), present in circulating blood plasma, contains information about prenatal health, organ transplant reception, and cancer presence and progression. Originally developed for the genomic analysis of highly degraded ancient DNA, single-stranded DNA (ssDNA) library preparation methods are gaining popularity in the field of cfDNA analysis due to their efficiency and ability to convert short, fragmented DNA into sequencing libraries without altering DNA ends. However, current ssDNA methods are costly and time-consuming.

Results

Here we present an efficient ligation-based single-stranded library preparation method that is engineered to produce complex libraries in under 2.5 h from as little as 1 nanogram of input DNA without alteration to the native

SUBMITTER: Troll CJ 

PROVIDER: S-EPMC6935139 | biostudies-literature | 2019 Dec

REPOSITORIES: biostudies-literature