Roles of the highly conserved amino acids in the second receptor binding site of the Newcastle disease virus HN protein.

ABSTRACT: BACKGROUND:The paramyxovirus haemagglutinin-neuraminidase (HN) is a multifunctional protein that is responsible for attachment to receptors, removal of receptors from infected cells to prevent viral self-aggregation (neuraminidase, NA) and fusion promotion. It is commonly accepted that there are two receptor binding sites in the globular head of HN, and the second receptor binding site is only involved in the function of receptor binding and fusion promotion. METHODS:10 conserved residues in the second receptor binding site of Newcastle disease virus (NDV) HN were chosen and substituted to alanine (A). The desired mutants were examined to detect the functional change in hemadsorption (HAD) ability, NA activity and fusion promotion ability. RESULTS:The HAD and fusion promotion ability of mu

SUBMITTER: Liu Y 

PROVIDER: S-EPMC6935236 | biostudies-literature | 2019 Dec

REPOSITORIES: biostudies-literature