CD206-positive myeloid cells bind galectin-9 and promote a tumor-supportive microenvironment.

ABSTRACT: In patients with metastatic melanoma, high blood levels of galectin-9 are correlated with worse overall survival and a bias towards a Th2 inflammatory state supportive of tumor growth. Although galectin-9 signaling through TIM3 on T cells has been described, less is known about the interaction of galectin-9 with macrophages. We aimed to determine whether galectin-9 is a binding partner of CD206 on macrophages and whether the result of this interaction is tumor-supportive. It was determined that incubation of CD68⁺ macrophages with galectin-9 or anti-CD206 blocked target binding and that both CD206 and galectin-9 were detected by immunoprecipitation of cell lysates. CD206 and galectin-9 had a binding affinity of 2.8 × 10^-7  m. Galectin-9 causes CD206⁺ macrop

SUBMITTER: Enninga EAL 

PROVIDER: S-EPMC6935353 | biostudies-literature | 2018 Aug

REPOSITORIES: biostudies-literature