Metagenomic Nanopore Sequencing of Influenza Virus Direct from Clinical Respiratory Samples.

ABSTRACT: Influenza is a major global public health threat as a result of its highly pathogenic variants, large zoonotic reservoir, and pandemic potential. Metagenomic viral sequencing offers the potential for a diagnostic test for influenza virus which also provides insights on transmission, evolution, and drug resistance and simultaneously detects other viruses. We therefore set out to apply the Oxford Nanopore Technologies sequencing method to metagenomic sequencing of respiratory samples. We generated influenza virus reads down to a limit of detection of 10² to 10³ genome copies/ml in pooled samples, observing a strong relationship between the viral titer and the proportion of influenza virus reads (P = 4.7 × 10^-5). Applying our methods to clinical throat

SUBMITTER: Lewandowski K 

PROVIDER: S-EPMC6935926 | biostudies-literature | 2019 Dec

REPOSITORIES: biostudies-literature