Inactivation of FOXO1 induces T follicular cell polarization and involves angioimmunoblastic T cell lymphoma.

ABSTRACT:

Objective

Angioimmunoblastic T cell lymphoma (AITL) is an aggressive form of non-Hodgkin lymphoma derived from mature T cells. However, the underlying pathogenesis of AITL remains unresolved. We aimed to explore the role of FOXO1-mediated signaling in the tumorigenesis and progression of AITL.

Methods

FOXO1 expression was assessed using immunohistochemistry on a total of 46 AITL tissue samples. Retroviruses encoding FOXO1 shRNA were used to knockdown FOXO1 expression in CD4⁺ T cells. Flow cytometric assays analyzed the proliferation and survival of FOXO1 knockdown CD4⁺ T cells. Furthermore, we performed adoptive T-cell transfer experiments to identify whether inactivation of FOXO1 induced neoplastic follicular-helper T (Tfh) cell polarization and functi

SUBMITTER: Xu M 

PROVIDER: S-EPMC6936234 | biostudies-literature | 2019 Nov

REPOSITORIES: biostudies-literature