Identification of four unconventional kinetoplastid kinetochore proteins KKT22-25 in Trypanosoma brucei.

ABSTRACT: The kinetochore is a multi-protein complex that drives chromosome segregation in eukaryotes. It assembles onto centromere DNA and interacts with spindle microtubules during mitosis and meiosis. Although most eukaryotes have canonical kinetochore proteins, kinetochores of evolutionarily divergent kinetoplastid species consist of at least 20 unconventional kinetochore proteins (KKT1-20). In addition, 12 proteins (KKT-interacting proteins 1-12, KKIP1-12) are known to localize at kinetochore regions during mitosis. It remains unclear whether KKIP proteins interact with KKT proteins. Here, we report the identification of four additional kinetochore proteins, KKT22-25, in Trypanosoma brucei. KKT22 and KKT23 constitutively localize at kinetochores, while KKT24 and KKT25 localize from S pha

SUBMITTER: Nerusheva OO 

PROVIDER: S-EPMC6936259 | biostudies-literature | 2019 Dec

REPOSITORIES: biostudies-literature