Evolution of resistance in vitro reveals mechanisms of artemisinin activity in Toxoplasma gondii.

ABSTRACT: Artemisinins are effective against a variety of parasites and provide the first line of treatment for malaria. Laboratory studies have identified several mechanisms for artemisinin resistance in Plasmodium falciparum, including mutations in Kelch13 that are associated with delayed clearance in some clinical isolates, although other mechanisms are likely involved. To explore other potential mechanisms of resistance in parasites, we took advantage of the genetic tractability of Toxoplasma gondii, a related parasite that shows moderate sensitivity to artemisinin. Resistant populations of T. gondii were selected by culture in increasing concentrations and whole-genome sequencing identified several nonconservative point mutations that emerged in the population and were fixe

SUBMITTER: Rosenberg A 

PROVIDER: S-EPMC6936365 | biostudies-literature | 2019 Dec

REPOSITORIES: biostudies-literature