Molecular profiling of single neurons of known identity in two ganglia from the crab Cancer borealis.

ABSTRACT: Understanding circuit organization depends on identification of cell types. Recent advances in transcriptional profiling methods have enabled classification of cell types by their gene expression. While exceptionally powerful and high throughput, the ground-truth validation of these methods is difficult: If cell type is unknown, how does one assess whether a given analysis accurately captures neuronal identity? To shed light on the capabilities and limitations of solely using transcriptional profiling for cell-type classification, we performed 2 forms of transcriptional profiling-RNA-seq and quantitative RT-PCR, in single, unambiguously identified neurons from 2 small crustacean neuronal networks: The stomatogastric and cardiac ganglia. We then combined our knowledge of cell type with unbi

SUBMITTER: Northcutt AJ 

PROVIDER: S-EPMC6936480 | biostudies-literature | 2019 Dec

REPOSITORIES: biostudies-literature