Approaching infinite affinity through engineering of peptide-protein interaction.

ABSTRACT: Much of life's complexity depends upon contacts between proteins with precise affinity and specificity. The successful application of engineered proteins often depends on high-stability binding to their target. In recent years, various approaches have enabled proteins to form irreversible covalent interactions with protein targets. However, the rate of such reactions is a major limitation to their use. Infinite affinity refers to the ideal where such covalent interaction occurs at the diffusion limit. Prototypes of infinite affinity pairs have been achieved using nonnatural reactive groups. After library-based evolution and rational design, here we establish a peptide-protein pair composed of the regular 20 amino acids that link together through an amide bond at a rate approaching the diff

SUBMITTER: Keeble AH 

PROVIDER: S-EPMC6936558 | biostudies-literature | 2019 Dec

REPOSITORIES: biostudies-literature