Comprehensive analysis of a mouse model of spontaneous uveoretinitis using single-cell RNA sequencing.

ABSTRACT: Autoimmune uveoretinitis is a significant cause of visual loss, and mouse models offer unique opportunities to study its disease mechanisms. Aire^-/- mice fail to express self-antigens in the thymus, exhibit reduced central tolerance, and develop a spontaneous, chronic, and progressive uveoretinitis. Using single-cell RNA sequencing (scRNA-seq), we characterized wild-type and Aire^-/- retinas to define, in a comprehensive and unbiased manner, the cell populations and gene expression patterns associated with disease. Based on scRNA-seq, immunostaining, and in situ hybridization, we infer that 1) the dominant effector response in Aire^-/- retinas is Th1-driven, 2) a subset of monocytes convert to either a macrophage/microglia s

SUBMITTER: Heng JS 

PROVIDER: S-EPMC6936593 | biostudies-literature | 2019 Dec

REPOSITORIES: biostudies-literature