A versatile modular vector set for optimizing protein expression among bacterial, yeast, insect and mammalian hosts.

ABSTRACT: We have developed a unified, versatile vector set for expression of recombinant proteins, fit for use in any bacterial, yeast, insect or mammalian cell host. The advantage of this system is its versatility at the vector level, achieved by the introduction of a novel expression cassette. This cassette contains a unified multi-cloning site, affinity tags, protease cleavable linkers, an optional secretion signal, and common restriction endonuclease sites at key positions. This way, genes of interest and all elements of the cassette can be switched freely among the vectors, using restriction digestion and ligation without the need of polymerase chain reaction (PCR). This vector set allows rapid protein expression screening of various hosts and affinity tags. The reason behind this approach was

SUBMITTER: Somogyi M 

PROVIDER: S-EPMC6936851 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature