Characterization and engineering of broadly reactive monoclonal antibody against hepatitis B virus X protein that blocks its interaction with DDB1.

ABSTRACT: Hepatitis B virus (HBV) X protein (HBx) plays diverse roles in both viral life cycle and HBV-related carcinogenesis. Its interaction with DNA damage-binding protein 1 (DDB1) was shown to be essential for engendering cellular conditions favorable for optimal viral transcription and replication. Previously, we described a mouse monoclonal antibody against HBx (anti-HBx 2A7) recognizing HBx encoded by representative strains from 7 of 8 known HBV genotypes. In this work, we further characterized 2A7 in order to explore its potential usefulness in HBx-targeting applications. We demonstrated that 2A7 recognizes a linear epitope mapped to L⁸⁹PKVLHKR⁹⁶ on HBx, a segment that is highly conserved across genotypes and coincidentally overlaps with the DDB1-interacting segment. HB

SUBMITTER: Tao S 

PROVIDER: S-EPMC6937242 | biostudies-literature | 2019 Dec

REPOSITORIES: biostudies-literature