Nervous system-wide profiling of presynaptic mRNAs reveals regulators of associative memory.

ABSTRACT: Presynaptic protein synthesis is important in the adult central nervous system; however, the nervous system-wide set of mRNAs localized to presynaptic areas has yet to be identified in any organism. Here we differentially labeled somatic and synaptic compartments in adult C. elegans with fluorescent proteins, and isolated synaptic and somatic regions from the same population of animals. We used this technique to determine the nervous system-wide presynaptic transcriptome by deep sequencing. Analysis of the synaptic transcriptome reveals that synaptic transcripts are predicted to have specialized functions in neurons. Differential expression analysis identified 542 genes enriched in synaptic regions relative to somatic regions, with synaptic functions conserved in higher organisms. We find

SUBMITTER: Arey RN 

PROVIDER: S-EPMC6937282 | biostudies-literature | 2019 Dec

REPOSITORIES: biostudies-literature