Oncotype DX Breast Cancer recurrence score resists inter-assay reproducibility with RT²-Profiler Multiplex RT-PCR.

ABSTRACT: The Oncotype Dx assay is frequently used to test if breast cancer patients can be spared from chemotherapy without negative effects for their future clinical course. However, due to conflicting data on the assay utility, in the recent past its reimbursement situation in Germany was revised; due to continued requests by clinicians for predictive values, our group decided to implement an Oncotype Dx like alternative assay with the objective of obtaining quality and cost optimization. Customized RT²-Profiler assays covering the 21 gene panel of the Oncotype Dx assay were applied to a pilot cohort of breast cancer patients with known Oncotype Dx Recurrence Score (RS). The Ct values obtained with RT²-Profiler-assays were used to calculate the unscaled Recurrence Score (RSu

SUBMITTER: Schildgen V 

PROVIDER: S-EPMC6937305 | biostudies-literature | 2019 Dec

REPOSITORIES: biostudies-literature