The sensor kinase MtrB of Mycobacterium tuberculosis regulates hypoxic survival and establishment of infection.

ABSTRACT: Paired two-component systems (TCSs), having a sensor kinase (SK) and a cognate response regulator (RR), enable the human pathogen Mycobacterium tuberculosis to respond to the external environment and to persist within its host. Here, we inactivated the SK gene of the TCS MtrAB, mtrB, generating the strain ΔmtrB We show that mtrB loss reduces the bacterium's ability to survive in macrophages and increases its association with autophagosomes and autolysosomes. Notably, the ΔmtrB strain was markedly defective in establishing lung infection in mice, with no detectable lung pathology following aerosol challenge. ΔmtrB was less able to withstand hypoxic and acid stresses and to form biofilms and had decreased viability under hypoxia. Transcriptional prof

SUBMITTER: Banerjee SK 

PROVIDER: S-EPMC6937564 | biostudies-literature | 2019 Dec

REPOSITORIES: biostudies-literature