Application of LpxC enzyme inhibitor to inhibit some fast-growing bacteria in human gut bacterial culturomics.

ABSTRACT: BACKGROUND:Culturomics can ascertain traces of microorganisms to be cultivated using different strategies and identified by matrix-assisted laser desorption/ionization-time-of-flight mass spectrometry or 16S rDNA sequencing. However, to cater to all requirements of microorganisms and isolate as many species as possible, multiple culture conditions must be used, imposing a heavy workload. In addition, the fast-growing bacteria (e.g., Escherichia) surpass the slow-growing bacteria in culture by occupying space and using up nutrients. Besides, some bacteria (e.g., Pseudomonas) suppress others by secreting antibacterial metabolites, making it difficult to isolate bacteria with lower competence. Applying inhibitors to restrain fast-growing bacteria is one method to cultivate more bacterial spec

SUBMITTER: Hou F 

PROVIDER: S-EPMC6937742 | biostudies-literature | 2019 Dec

REPOSITORIES: biostudies-literature