Enhancer polymorphism rs10865710 associated with traumatic sepsis is a regulator of PPARG gene expression.

ABSTRACT:

Background

Peroxisome proliferator-activated receptor gamma (PPARγ) is a major regulator in sepsis. Our previous study identified the enhancer polymorphism rs10865710C/G to be associated with susceptibility to sepsis in trauma patients. We performed two-stage cohort studies integrating biological experiments of potential functional variants that modify susceptibility to traumatic sepsis.

Methods

Improved multiplex ligation detection reaction (iMLDR) was used to genotype rs10865710 in 797 Han Chinese trauma patients in Chongqing. Clinical relevance was validated in 334 patients in Guizhou. The potential function of rs10865710 in transcriptional regulation was explored through a dual luciferase reporter assay and electrophoretic mobility shift assay (EMSA). Expression of PPARγ

SUBMITTER: Lu H 

PROVIDER: S-EPMC6938012 | biostudies-literature | 2019 Dec

REPOSITORIES: biostudies-literature