High-resolution lineage tracking reveals travelling wave of adaptation in laboratory yeast.

ABSTRACT: In rapidly adapting asexual populations, including many microbial pathogens and viruses, numerous mutant lineages often compete for dominance within the population^1-5. These complex evolutionary dynamics determine the outcomes of adaptation, but have been difficult to observe directly. Previous studies have used whole-genome sequencing to follow molecular adaptation^6-10; however, these methods have limited resolution in microbial populations. Here we introduce a renewable barcoding system to observe evolutionary dynamics at high resolution in laboratory budding yeast. We find nested patterns of interference and hitchhiking even at low frequencies. These events are driven by the continuous appearance of new mutations that modify the fates of existing lineages before th

SUBMITTER: Nguyen Ba AN 

PROVIDER: S-EPMC6938260 | biostudies-literature | 2019 Nov

REPOSITORIES: biostudies-literature