Cytotoxicity Evaluation of Minimum Antibacterial Values of Different Medicaments Used in Endodontic Regenerative Procedures.

ABSTRACT:

Objectives

 This study aimed to evaluate the cytotoxicity of minimum antibacterial values of medicaments used in endodontic regeneration on stem cells.

Materials and methods

 "Minimum inhibitory concentration," "minimum bactericidal concentration," and "minimum biofilm inhibitory concentration" of triple and double antibiotic paste, a modified triple antibiotic paste (minocycline replaced by clindamycin), Augmentin, and calcium hydroxide were determined using Enterococcus faecalis (ATCC 29212) by microtiter plate method. Direct cytotoxic effects of drugs were evaluated by lactate dehydrogenase and water-soluble tetrazolium salt-1 assays using stem cells of apical papilla obtained from immature third molars via enzymatic digestion.

Statistical analysis

 Data wer

SUBMITTER: Khoshkhounejad M 

PROVIDER: S-EPMC6938419 | biostudies-literature | 2019 Oct

REPOSITORIES: biostudies-literature