CD34 and EPCR coordinately enrich functional murine hematopoietic stem cells under normal and inflammatory conditions.

ABSTRACT: Hematopoiesis is dynamically regulated to maintain blood system function under nonhomeostatic conditions such as inflammation and injury. However, common surface marker and hematopoietic stem cell (HSC) reporter systems used for prospective enrichment of HSCs have been less rigorously tested in these contexts. Here, we use two surface markers, EPCR/CD201 and CD34, to re-analyze dynamic changes in the HSC-enriched phenotypic SLAM compartment in a mouse model of chronic interleukin (IL)-1 exposure. EPCR and CD34 coordinately identify four functionally and molecularly distinct compartments within the SLAM fraction, including an EPCR⁺/CD34^- fraction whose long-term serial repopulating activity is only modestly impacted by chronic IL-1 exposure, relative to unfractionated

SUBMITTER: Rabe JL 

PROVIDER: S-EPMC6938677 | biostudies-literature | 2020 Jan

REPOSITORIES: biostudies-literature