Kinetic data analysis of chaperone-like activity of Wt, R69C and D109H αB-crystallins.

ABSTRACT: The α-Crystallin (α-Cry) functions as a molecular chaperone, preventing the formation of stress-induced protein aggregation which is important for maintenance of lens transparency. The kinetic data of Wt, R69C and D109H αB-Crys chaperone-like activity were obtained by UV-Vis spectroscopy in both thermal- and chemical-induced aggregation methods. The data were analyzed using physical parameters describing the aggregation process including t* (the characteristic of the stage of nucleation), and t _0.5 (the characteristic of the stage of aggregate growth) and I _lim (the limiting value of the light scattering intensity). Parameter t* is duration of the lag phase and the lower t* value is associated with the higher rate of the nucleation s

SUBMITTER: Ghahramani M 

PROVIDER: S-EPMC6939022 | biostudies-literature | 2020 Feb

REPOSITORIES: biostudies-literature